ELISA Kit for Hepatitis B Surface Antigen
(One-step Double Antibody Sandwich immunoassay)
INTENDED USE
The Kinghawk HBsAg ELISA Kit is a double antibody sandwich ELISA designed to detect HBsAg in human serum or plasma. It is used for blood screening and aided diagnosis for Hepatitis B virus infection.
PRINCIPLE
The anti-HBsAg monoclonal is coated on the solid phase of multi-wells. Serum sample and multiclonal anti-HBs (conjugated) are added to coated wells. Incubate with TMB to form a colored product or not to indicate the presence or absence of HbsAg.
REAGENTS
Detect HBsAg Microplate | 1 *96wells |
Enzyme Conjugate (peroxidase labeled Second Antibody) | 1 vial (7ml) |
Positive Control | 1 vial (1ml) |
Negative Control | 1 vial (1ml) |
Wash Buffer (20×) | 1 vial (30ml) |
Peroxidase Substrate Buffer | 1 vial (7ml) |
TMB Substrate | 1 vial (7ml) |
Stop Solution | 1 vial (7ml) |
NOTE: Kit also contains: Two Plate Sealers/One self-seal plastic bag
/One User Guide
SPECIMEN REQUEST
1. Fresh Serum or plasma sample is suitable for this kit (anticoagulant like Sodium Citrate,heparin,etc.)
2. Not test severe hemolytic sample or the sample containing sodium azide.
3. Before testing, the sample should be balanced to room temperature (15~30°C), frozen sample need to thaw and adequately mix.
4. If the sample will not be tested immediately after collection, should be stored at 2~8°C for 3 days. Long-term storage should be placed at -20°C or lower temperature.
5. No repeated freezing and thawing sample as well as heating sample be used.
PACKAGE: 96T
STORAGE: The kit must be stored at 2~8ºC Do not freeze Conjugate
SHELF LIFE: 12months
BEIJING KINGHAWK PHARMACEUTICAL CO., LTD